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An overview of the test procedure is provided below:

1. Collect urine sample

collect urine sample


2. Open the tube containing the test reagent

open tube


3. Using the pipet provided with the kit, add a small volume of urine to the test reagent




4. Mix by gently shaking the tube and wait for three minutes

gently shake


5. Observe color changes

observe color changes


For negative samples, there is no change in color (first two tubes). Third and fourth tubes correspond to moderate positives. Last two tubes are strong positives.


The manufacturer has provided the following FAQ

What does the test measure, what does it mean?

The Neurotoxic Metabolite Test aims at detecting the presence of specific metabolites in the urine. These metabolites are related to the production of hydrogen sulfide (H2S). Although H2S is naturally present in the body, and plays some normal physiological functions, an excess production can be very detrimental. Overproduction of H2S may result from metabolic dysfunctions, but also from the overgrowth of certain bacteria in the gut (alteration of the normal intestinal flora = gut dysbiosis). Our preliminary results indicate that a strong proportion of Chronic Fatigue Syndrome/Myalgic Encephalomyelitis(CFS/ME) patients present such dysbiosis, and test positive with the NMT test; but positivity may be associated with other conditions, related to intestinal dysfunction

Is this kit recognized as a diagnostic kit?

NO. We are in the process of getting accreditation (CE mark) but this will take several months. Meanwhile, due to the strong need expressed by the patient community, we decided to make it available as a “research use only”. When signing the ordering forms, people who order the kit recognize that this is not a diagnostic kit. Because the kit is not officially approved we have to state that it is not intended to diagnose or prevent any disease.

Can medications/supplementations affect the result?

We have not yet completed all studies to find out the effects of medications on the test result. Since gut microflora is a major factor determining H2S production, it is very likely that antibiotic use, or concentrated probiotic consumption (but not the basic probiotics found in some dairy products), will have an effect on the test result. We have not yet studied the effect of pregnancy on the test result, but it is unlikely to affect the outcome of the test.

However, we certainly do not recommend that you stop a treatment which was prescribed by a health care provider. Just be aware that it may affect the test result, or wait until your treatment is finished before taking the test.

How many analyses can I do with one kit?

 Each kit allows to do one analysis


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